Year 2025, Volume 71 Issue 2
| Year : | 2025 |
| Volume : | 71 |
| Issue : | 2 |
| Authors : | Hasan KOUCHACKKHANI, Mohsen JANMOHAMMADI, Asghar EBADI-SEGHERLOO, Naser SABAGHNIA |
| Title : | THE EFFECT OF PLANT DENSITY, VARIOUS ORGANIC AND NANO-FERTILIZERS ON ACCUMULATION AND ACTIVITY OF PROTECTIVE MOLECULES IN CARTHAMUS TINCTORIUS L. |
| Abstract : | Agronomic management such as plant density and plant nutrition can affect the final performance of the plant through the alternation in molecular activity. A field experiment was designed to evaluate the effect of fertilizers (C: control, AM10, and AM20: application of 10 and 20 t ha-1 of animal manure, NPK, nano Fe+Zn fertilizers) and inter-row spacing (IS: 40 and 60 cm) on the accumulation and activity of some protective molecules in safflower plants in the Hamadan region-west of Iran. The application of all fertilizers significantly increased catalase activity compared to control conditions. Planting in narrower rows and applying nano Fe+Zn resulted in the highest ascorbate peroxidase activity and the lowest activity was recorded under NPK + IS60. The consumption of micronutrients increased the activity of guaiacol peroxidase about 3 times compared to the control. The highest activity of total superoxide dismutase was obtained by using NPK+IS60. The highest amount of protein in leaves was recorded in AM20 + IS60 and NPK+IS60 application conditions. The lowest amount of hydrogen peroxide was observed in plants grown under AM10 + IS60 conditions. Consumption of AM20 reduced the level of malondialdehyde by 42% compared to the control condition. However, the consumption of high levels of animal manure decreased the amount of proline in the leaves by 2 times compared to the control. Altogether, the results showed that lower planting density and the use of high levels of organic fertilizer, NPK, and nanostructured micronutrients had a significant effect on increasing antioxidant activity and improving the defense function of safflower |
| For citation : | Kouchackkhani, H., Janmohammad, M., Ebadi-Segherloo, A., Sabaghnia, N. (2025). The effect of plant density and nutrition on accumulation and activity of protective molecules in Carthamus tinctorius L. Agriculture and Forestry, 71 (2): 107-121. https://doi:10.17707/AgricultForest.70.4.07 |
| Keywords : | antioxidants, ascorbate peroxidase, catalase, guaiacol peroxidase, proline |
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