The Journal "Agriculture and Forestry"
Časopis "Poljoprivreda i šumarstvo"
- Since 1955 -
Year 2025, Volume 71 Issue 1
| Year : | 2025 |
| Volume : | 71 |
| Issue : | 1 |
| Authors : | Yuriy E. KOLUPAEV, Alexander I. KOKOREV, Liubov N. KOBYZEVA, Tamara V. SAKHNO, Olha BARABOLIA, Tetiana O. YASTREB |
| Title : | PRIMING WITH NO DONOR SODIUM NITROPRUSSIDE TO ACTIVATE GERMINATION AND REDUCE OXIDATIVE DAMAGE IN AGED WHEAT AND TRITICALE SEEDS |
| Abstract : | Despite the existence of numerous experimental data on the stimulation of seed germination of various plant species under unfavourable conditions by nitric oxide donors, there are very few data on the effect of exogenous NO on the germination of cereal seeds that have undergone accelerated ageing. In particular, they are lacking for aged seeds of one of the most widespread bread cereals, Triticum aestivum L. Any physiological effect of NO on the wheat-rye hybrid triticale (×Triticosecale) has not been studied. The aim of this work was to study the effect of priming aged wheat seeds of cultivar Scorpion and triticale seeds of cultivar Raritet with the nitric oxide donor sodium nitroprusside (SNP) on their germination and seedling growth parameters. A three-hour treatment of seeds with SNP (optimum concentration 100 µM) resulted in increased germination energy, germination, and accumulation of shoot and root biomass in both species. The effects of SNP were almost completely eliminated by the action of the NO scavenger methylene blue. Moreover, there was no effect of SNP on seed germination and seedling growth subsequent to its preliminary decomposition under light conditions. This suggests that the physiological activity of SNP may be linked to the release of nitrogen oxide. Treatment of seeds with SNP resulted in a reduction in the generation of superoxide anion radicals and hydrogen peroxide in the shoots of seedlings of both species, as well as a reduction in the content of malonic dialdehyde, a product of lipid peroxidation. Concomitantly, alterations in the activity of pivotal antioxidant enzymes (superoxide dismutase and catalase) were observed in seedlings derived from seeds treated with SNP. Furthermore, the application of SNP to seeds resulted in an increase in the total phenolic compound content in wheat seedlings and the amount of anthocyanins in triticale seedlings. It was concluded that there is a correlation between the ability of exogenous NO to mitigate oxidative damage and enhance germination of aged cereal seeds. |
| For citation : | Kolupaev, Y.E., Kokorev, A.I., Kobyzeva, L.N., Sakhino, T.V., Barabolia, O., Yastreb, T.O., (2025). Priming with no donor sodium nitroprusside to activate germination and reduce oxidative damage in aged wheat and triticale seeds. Agriculture and Forestry, 71 (1): 07-26. https://doi:10.17707/AgricultForest.71.1.01 |
| Keywords : | Triticum aestivum, × Triticosecale, seed aging, germination, nitric oxide, oxidative stress, antioxidant system |
| download paper | |