The Journal "Agriculture and Forestry"
Časopis "Poljoprivreda i šumarstvo"
- Since 1955 -
Year 2018, Volume 64 Issue 4 (15.12.2018)
| Year : | 2018 |
| Volume : | 64 |
| Issue : | 4 (15.12.2018) |
| Authors : | Lidiya MISHCHENKO, Alina DUNICH, Ivan MISHCHENKO, Olga MOLODCHENKOVA |
| Title : | MOLECULAR AND BIOLOGICAL PROPERTIES OF SOYBEAN MOSAIC VIRUS AND ITS INFLUENCE ON THE YIELD AND QUALITY OF SOYBEAN UNDER CLIMATE CHANGE CONDITIONS |
| Abstract : | Soil and climatic conditions of Ukraine provide the obtaining of ecologically sound yields of grain crops and legumes, among which the soybean occupies the forefront. In 2017, the average soybean yield was significantly (by 3.4 - 13 centner/ha) lower than in 2016. The aim of the work was to investigate the molecular and biological properties of the Soybean mosaic virus (SMV) isolate SKP-16, its effect on soybean yield and grain quality under agro-climatic change conditions. In 2015-2017, monitoring inspections of soybean crops showed that viral infections, occupied a significant place, mainly SMV. Also, seed transmission of SMV infected several soybean varieties was proved. In 2017, a very dry year for Ukraine, and especially for Poltava region, we found a significant infecting of many varieties with SMV. Hydrothermal coefficient of G.T. Selyaninov (HTC) was less than 1, only 0.53. This indicator takes into account simultaneously the actions of the main climate elements – temperature and precipitation. The low HTC provided a significant number of aphids – vectors of SMV. We have studied in detail the SMV isolate SKP-16 from soybean variety Kophy (GenBank Accession No MG940990). The yield of soybeans in the SMV-infected plants was reduced by 2.6 times, compared with healthy ones in 2017. But in 2016, the difference was considerably smaller, indicating a synergistic harmful effect of both factors - the virus and climatic conditions. The analysis of the nucleotide and amino acid sequences of the SKP-16 capsid protein gene revealed the highest percentage of identity (97.9% and 97.2% respectively) with the isolates UA1Gr, Ar33, Lo3, VA2. Four amino acid substitutions were found in position 1 (Ser → Cys), position 2 (Lys → Ser), position 3 (Gly → Leu), and position 5 (Val → Leu). |
| For citation : | Mishchenko, L., Dunich, A., Mishchenko, I., Molodchenkova, O. (2018): Molecular and biological properties of Soybean mosaic virus and its influence on the yield and quality of soybean under climate change conditions. Agriculture and Forestry, 64 (4): 39-47. DOI:10.17707/AgricultForest.64.4.05 |
| Keywords : | soybean, Soybean mosaic virus, sequencing, yield, quality |
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