The Journal "Agriculture and Forestry"
Časopis "Poljoprivreda i šumarstvo"
- Since 1955 -
Year 2018, Volume 64 Issue 3 (30.09.2018)
| Year : | 2018 |
| Volume : | 64 |
| Issue : | 3 (30.09.2018) |
| Authors : | Khemais ABDELLAOUI, Fatma ACHEUK, Meriem MILADI, Iteb BOUGHATTAS, Ghofrane OMRI |
| Title : | PHYTOCHEMISTRY, BIOCHEMICAL AND INSECTICIDAL ACTIVITIES OF RUTA CHALEPENSIS ESSENTIAL OILS ON TRIBOLIUM CONFUSUM |
| Abstract : | The confused flour beetle, Tribolium confusum (Coleoptera: Tenebrionidae) is a common pest insect known for attacking and infesting stored flour and grain. Biodegradable and ecologically natural products such as essential oils are emerging candidates for replacement of usually applied chemical pesticides. The essential oils of Ruta chalepensis flowering aerial parts were investigated for their contact toxicity and physiological aspects on T. confusum. Essential oils were obtained by hydrodistillation and 56 components were identified by GC-MS. Our results clearly indicated that these compounds exhibited toxicity against T. confusum pupae and adults with an LC50 value of 0.08 and 0.055 µL/cm2, respectively after 7 days of treatment. In repellency assay, essential oils repelled T. confusum adults significantly even at 0.06 µL/cm2 concentration in an area preference test. Result also showed that maximum exposure time resulted in maximum repellency of the pest at all the concentrations. The repellent activity could be related to the abundance of the 2-undecanone (25.94%) in the oils. In other experiments, the essential oils were investigated on the activities of acetylcholinesterase (AChE) and glutathione S-transferases (GSTs). Biochemical analysis demonstrated that the essential oils of R. chalepensis induced GSTs and reduced the activity of AChE. Based on these results, essential oils of R. chalepensis origin could have greater potential in future in stored-product pest management. |
| For citation : | Abdellaoui, K., Acheuk, F., Miladi, M., Boughattas, I., Omri, G. (2018): Phytochemistry, biochemical and insecticidal activities of Ruta chalepensis essential oils on Tribolium confusum. Agriculture and Forestry, 64 (3): 31-45. DOI:10.17707/AgricultForest.64.3.03 |
| Keywords : | Essential oils, phytochemistry, toxicity, Acetylcholinesterase, glutathione S-transferases |
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