The Journal "Agriculture and Forestry"
Časopis "Poljoprivreda i šumarstvo"
- Since 1955 -
Year 2017, Volume 63 Issue 3 (30.09.2017)
| Year : | 2017 |
| Volume : | 63 |
| Issue : | 3 (30.09.2017) |
| Authors : | Hamed HASSANZADEH KHANKAHDANI, Somayeh RASTEGAR, Behrooz GOLEIN, Morteza GOLMOHAMMADI and Abdolhossein ABOUTALEBI JAHROMI |
| Title : | GENETIC DIVERSITY IN PERSIAN LIME (CITRUS LATIFOLIA TANAKA) ACCESSIONS USING MORPHOLOGICAL AND MOLECULAR MARKERS |
| Abstract : | Persian lime (PL), Citrus latifolia Tanaka, is a triploid and WBDL (Witches Broom Disease of lime) resistant lime which experiences cultivation extension these days. In addition to PL, there are many unknown triploid lime germplasms in the south of Iran, which necessitate to be identified. The current study was conducted to evaluate phylogenetic relationships among PL accessions in Iran. To do so, eighteen known and unknown genotypes of PL and Mexican lime, collected from Fars, Hormozgan and Mazandaran provinces of Iran, as well as eight systematically known citrus varieties were studied by ISSR and morphological markers. Eight ISSR primers produced 139 polymorphic bands in which polymorphic Information Content (PIC) varied from 0.384 to 0.499. The morphological study divided examined accessions into two main groups including control accessions (Pummelo, Citron, Lisbon lemon, Sweet lime, Grapefruit and Dancy) and twenty lime and lemon accessions. Both morphological and ISSR markers produced a clear-cut resolution among genotypes and could separate the control accessions from the other ones. Molecular inferred tree grouped all examined accessions and verities into 7 clades in which Mexican lime accessions (Mexican lime, IFJKh, IFJKMes and Cucumber-shaped lime) and PL accessions grouped in two close groups. As we expected, Persian and Mexican lime accessions showed genetically close relation. The most striking result to emerge from the data was highly correlation between morphological and ISSR data |
| For citation : | Hassanzadeh Khankahdani H., Rastegar S., Golein B., Golmohammadi M. And Aboutalebi Jahromi A., (2017) Genetic diversity in persian lime (Citrus latifolia Tanaka) accessions using morphological and molecular markers, Agriculture and Forestry, 63 (3): 221-231. DOI: 10.17707/AgricultForest.63.3.22 |
| Keywords : | Deperse lime, IAC, Bearss lime, Microsatellites, Tahiti lime |
| download paper | |