The Journal "Agriculture and Forestry"
Časopis "Poljoprivreda i šumarstvo"
- Since 1955 -
Year 2017, Volume 63 Issue 3 (30.09.2017)
| Year : | 2017 |
| Volume : | 63 |
| Issue : | 3 (30.09.2017) |
| Authors : | Majid BAGNAZARI, Mehdi SAIDI, Prakash HARISHCHANDRA SRIPATHY, Geetha NAGARAJA |
| Title : | ESTABLISHMENT OF AN IMPROVED, EFFICIENT AND ECO-FRIENDLY MICROPROPAGATION SYSTEM IN SALACIA CHINENSIS L. AN ENDANGERED ANTI-DIABETIC MEDICINAL PLANT |
| Abstract : | An efficient micropropagation system via indirect organogenesis was developed in Salacia chinensis L., an endangered anti-diabetic medicinal plant. Accurate investigation of the various plant sources (Leaf, node, shoot tip) and plant growth regulators (PGRs) impacts were accomplished in this study. Maximum rate of callus induction (93.43 ± 2.75%) was achieved from nodes inoculated on MS medium fortified with 1.0 mg/l NAA+ 2.0 mg/l BAP. Maximum shoot induction (93.33 ± 2.02%), number of shoots/explant (5.12 ± 0.09) and shoot length (3.17 ± 0.00 cm) were obtained from nodal explants inoculated on MS medium with 1.5 mg/l BAP + 1.0 mg/l NAA. IBA (2.0 mg/l) in ½ MS medium was observed to be the best rooting treatment, which promoted the highest frequency of rooting (91.66 ± 2.33%). The results suggested an efficient regeneration system for conservation and large scale production for pharmaceutical industry demands |
| For citation : | Bagnazari M., Saidi M., Harishchandra Sripathy P., Nagaraja G., (2017) Establishment of an improved, efficient and eco-friendly micropropagation system in Salacia chinensis L. an endangered anti-diabetic medicinal plant, Agriculture and Forestry, 63 (3): 167-176. DOI: 10.17707/AgricultForest.63.3.17 |
| Keywords : | Salacia chinensis, Anti-diabetic, Medicinal plant, Indirect organogenesis, In vitro conservation |
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