The Journal "Agriculture and Forestry"
Časopis "Poljoprivreda i šumarstvo"
- Since 1955 -
Year 2017, Volume 63 Issue 1 (31.03.2017)
| Year : | 2017 |
| Volume : | 63 |
| Issue : | 1 (31.03.2017) |
| Authors : | Valentine BRAILKO, Olga MITROFANOVA, Nina LESNIKOVA-SEDOSHENKO, Svetlana CHELOMBIT, Irina MITROFANOVA |
| Title : | ANATOMY FEATURES OF LAVANDULA ANGUSTIFOLIA MILL. AND LAVANDULA HYBRIDA REV. PLANTS IN VITRO |
| Abstract : | Lavender and lavandin are widely used in food, pharmaceutical, cosmetic industries and ornamental gardening. Gene pool collection of Lavandula angustifolia and lavandin is presented in Nikita Botanical Gardens plots and the same collection is being created in vitro. Initial explants of L. angustifolia, its cultivars 'Belyanka', 'Record' and L. hybrida – 'Rabat' and 'Snezhnyi Bars' were introduced in vitro. Plant regeneration occurred on MS medium supplemented with 0.3-0.5 mg l-1 kinetin, 0.025 mg l-1 NAA, 0.25 mg l-1 GA3. Samples were analyzed after 2 and 8 months of culture. Structural analysis of regenerated plants vegetative organs enabled assessment of their morphogenetic capacity. Under in vitro culture 2-5 microshoots/explants were produced with the height of 2.3 - 8.2 cm. Leaves were opposite, sessile linear or linear-lanceolate, with entire edges curved outward, densely pubescent, and 10-30 leaves/microshoot. Leaf lamina length was 0.9-1.5 cm. Leaves were bifacial, amphystomatic, lamina thickness – 132-215 µm, palisade rate was 0.38-0.41 after 2 months culture and 0.56-0.58 – after 8 months. Epidermal thickness was 8-19 µm, cells were relatively small with thin, cellulose walls covered with cuticle. Stomata are of anomocyte type, small, raised above the epiderm cells, 48-150 pcs/mm2 on adaxial surface, 115-432 pcs/mm2 – on abaxial one. Essential oils are produced in simple and glandular, unicellular and multicellular hairs of epidermal origin, located above the leaf chlorophyll tissue. Features of xeromorphic structure appeared at a long-term in vitro cultivation. |
| For citation : | Brailko V., Mitrofanova O., Lesnikova-Sedoshenko N., Chelombit S., Mitrofanova I., (2017) Anatomy features of Lavandula angustifolia Mill., Agriculture and Forestry, 63 (1): 111-117. DOI: 10.17707/AgricultForest.63.1.13 |
| Keywords : | lavender, lavandin, regenerant, leaf anatomy, xeromorphic structure. |
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