The Journal "Agriculture and Forestry"
Časopis "Poljoprivreda i šumarstvo"
- Since 1955 -
Year 2017, Volume 63 Issue 1 (31.03.2017)
| Year : | 2017 |
| Volume : | 63 |
| Issue : | 1 (31.03.2017) |
| Authors : | Vida CHALAVI, Sodabeh ZAREH |
| Title : | GENETIC ENGINEERING OF ROSE SCENTED GERANIUM WITH BACTERIAL GENES FOR PHYTOREMEDISTION OF POLYCHLORINATED BIPHENYLD |
| Abstract : | Polychlorinated biphenyls (PCBs) are major water and soil contaminants worldwide. These harmful compounds to human health are highly stable and resistance to degradation. Biphenyl dioxygenase (BPDO) is a bacterial enzyme from Burkholderia xenovorans which has catabolic degradation activity of PCBs. In present study we report simultaneous genetic transformation of three coding components of BPDO enzyme,bphA, bphE, and bphG genes, into rose scented geranium (Pelargonum graveolens L.), a fast growing plant with high phytoremediation potential. Toward this end, the bphA, bphE, and bphG genes were cloned into pGreen0029 vector. Then, pGreen0029 vector carrying all three genes along with pSoup plasmid were introduced into Agrobacterium tumefaciens strain LBA4404 which then were used for transformation of rose scented geranium. Fully green putative transformant plantlets produced in selection medium were successfully rooted in presence of 50 mgl-1 kanamycin. The transgenic nature of fully green and rooted rose scented geranium plantlets were confirmed by Polymerase Chain Reaction (PCR) analysis. Specific primers of bphA gene (one of the three tandem cloned genes in the vector of this experiment) was used for PCR analysis and produced a 1380 bp fragment which is the exact size of this gene. Transgenic plants were successfully transferred into soil and continued their growth. |
| For citation : | Chalavi V., Zareh S., (2017) Genetic engineering of rose scented geranium with bacterial genes for phytoremedistion of polychlorinated biphenyld, Agriculture and Forestry, 63 (1): 35-40. DOI: 10.17707/AgricultForest.63.1.04 |
| Keywords : | chlorinated biphenyls, rose scented geranium, Agrobacterium LBA4404, pGreen vector. |
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