Year 2016, Volume 62 Issue 4 (15.12.2016)
Year : | 2016 |
Volume : | 62 |
Issue : | 4 (15.12.2016) |
Authors : | Hakan ENGIN and Zeliha GÖKBAYRAK |
Title : | IN VITRO POLLEN VIABILITY AND GERMINATION OF BISEXUAL AND FUNCTIONAL MALE FLOWERS OF SOME TURKISH POMEGRANATE CULTIVARS |
Abstract : | The evaluation of pollen viability and germination are two essential criteria for characterization of pollinating cultivars in pomegranate which is an andromonoecious species. With this point of view, pollens were collected from both bisexual and functional male flowers of ‘Aºınar’ and ‘Caner II’ cultivars to determine the pollen viability and germination. Colorimetric test of 2,3,5-triphenyl tetrazolium chloride (TTC) was used to estimate the pollen viability. Pollen germination was tested in vitro on a medium containing 1% agar and 20% sucrose. Results showed that pollen viability was not dependent on the type of the flowers. The highest pollen viability ratio (78%) was obtained in the pollen collected from functional male flowers of ‘Aºınar’. Pollen germination ratio, however, varied with regard to the flower type in the same cultivar. Bisexual and male flowers in ‘Aºınar’, respectively, had 43.5% vs 22.3% pollen grains germinated. Pollens from two sexual morphs of pomegranates show different germination and viability ratios. Investigation on viability and germination capability of pollen grains coming from both bisexual and functional male flowers in pomegranate can enable growers and breeders to better select best crossing pollinators. |
For citation : | Engin H., Gökbayrak Z. (2016): In vitro pollen viability and germination of bisexual and functional male flowers of some Turkish pomegranate cultivars Agriculture and Forestry, 62 (4):91-94. DOI: 10.17707/AgricultForest.62.4.11 |
Keywords : | Punica granatum L., Pollen, Viability, Germination. |
download paper |